The Unmet Need in Acute Respiratory Infections
Millions of people are currently at risk for acute respiratory infections (ARIs). These infections are the starting point for serious illnesses, such as bronchiolitis and pneumonia and can exacerbate underlying conditions, including COPD and congestive heart failure. For vulnerable populations including pediatric, elderly and immunocompromised, this issue has only become worse since the onset of the COVID-19 pandemic.
Still, despite decades of R&D, there has been limited success in treating ARIs. Without approved treatments, these conditions can lead to hospitalization, long-term health complications and even death.
Muco-Trapping Antibodies - A Breakthrough Approach
Inhalon Biopharma’s breakthrough platform for treating ARIs enables inhaled antibodies to trap viruses and other pathogens in mucus lining the airways, block the infection from spreading within the lung, and rapidly eliminate the germs through natural mucus clearance. This new "muco-trapping" immune function was made possible by key molecular and biophysical insights into antibody-mucin interactions, which allow antibodies to bind to pathogens and trap them in mucus by crosslinking to mucins, the mesh network that makes up mucus. Trapped viruses are carried out of the lung by natural ciliary beating or cough driven expulsion. This unique antibody function is broadly applicable to viruses, bacteria and fungi. Inhalon's muco-trapping technology is protected by four issued patents in the US and Europe and multiple patent applications throughout the world.
Our inhaled immunotherapy platform is especially suited for treating ARIs, such as respiratory syncytial virus (RSV), influenza, metapneumovirus (MPV), parainfluenza virus (PIV), adenovirus, coronaviruses, including severe acute respiratory syndrome coronavirus (e.g., SARS-CoV-2) and Middle East respiratory syndrome (MERS).
Why Muco-Trapping is Different
Inhalon’s platform halts the infection immediately, directly clears viruses and other pathogens from infected airways, blocks transmission, requires much less antibody compared to systemic dosing, and allows treatment at the comfort of a patient’s home.
How Muco-Trapping Works
Our breakthrough discovery enables inhaled antibodies to trap pathogens (i.e., viruses, bacteria, fungi) in the lung mucus, which in turn blocks the infections from further spreading locally and facilitates the rapid elimination of the pathogens from the lung through normal mucus clearance. Although mucus in the lung airways is designed to protect the body from infections, pathogens have evolved to move easily through mucus to infect cells in the airways. Inhalon was launched based on the discovery of a new immune function – antibodies trapping pathogens in mucus – and molecular and biophysical insights into how to tune the antibody-mucin affinity. These “muco-trapping” antibodies bind to pathogens and crosslink them to mucins. Trapped pathogens are unable to infect cells and are rapidly eliminated by normal mucus clearance every 30 to 60 minutes – swept into the digestive tract and become sterilized by gastric acid. This muco-trapping function is mediated by glycans in the antibody’s Fc region and enhanced through the normal glycosylation process using Inhalon-engineered production cell lines. This muco-trapping phenomenon has been confirmed with over a half dozen pathogens to date.
Inhalon's Platform Treats ARIs More Effectively
The videos below show how muco-trapping antibodies added to human mucus can trap bacteria and viruses so they can be swept out of the airways.
Muco-Trapping Antibody Added
Muco-Trapping Antibody Added
Patients are treated by inhaling an antibody mist that is generated by a handheld nebulizer. Inhalon uses vibrating nebulizers from third-parties to aerosolize the antibody drug without changing the antibody's properties. Other types of nebulizers can damage antibodies making them ineffective.
Inhalon's muco-trapping antibodies are designed to come in single-use containers that can easily be poured into the nebulizer. Patients inhale the antibody drug for 3 to 5 minutes using the nebulizer mouthpiece or mask. This process is repeated for 3 to 7 days depending on the indication.
Our Solution: Inhaled "Muco-Trapping" Antibodies
Battery Operated Nebulizer
Wang YY, Kannan A, Nunn KL, Murphy M, Subramani DB, Moench TM, Cone RA, Lai SK. (2014) IgG in cervicovaginal mucus traps HSV and prevents vaginal Herpes infections. Mucosal Immunology. 7(5):1036-44
Chen, A., S.A. McKinley, S. Wang, F. Shi, P.J. Mucha, M.G. Forest, and S.K. Lai, Transient antibodymucin interactions produce a dynamic molecular shield against viral invasion. Biophys J, 2014. 106(9): p. 2028-36.
Wessler T, Chen A, McKinley SA, Cone R, Forest MG, Lai SK. (2015) Using computational modeling to optimize the design of antibodies that trap viruses in mucus. ACS Infectious Disease. 2(1):82-92
Wang YY, Harit D, Subramani DB, Arora H, Kumar P, Lai SK. (2017) Influenza-binding antibodies immobilise influenza viruses in fresh human airway mucus. European Respiratory Journal 49:1601709.
Newby J, Schiller J, Wessler T, Edelstein J, Forest MG, Lai SK. (2017) A blueprint for robust crosslinking of mobile species in biogels using third-party molecular anchors with short-lived anchor-matrix bonds. Nature Communications. 8(1):833
Yang B, Schaefer A, Wang YY, McCallen JD, Lee P, Newby J, Arora H, Kumar PA, Zeitlin L, Whaley KJ, McKinley SA, Fischer W, Harit D, Lai SK. (2018) ZMapp Reinforces the Airway Mucosal Barrier Against Ebola Virus. Journal of Infectious Disease. 218(6):901-910
Zhu F, Jay M, Schiller JL, Schroeder HA, Wessler T, Chen A, Forest MG, Lai SK. (2019) Modeling barrier properties of intestinal mucus reinforced with IgG and secretory IgA against motile bacteria. ACS Infectious Disease. 5(9):1570-1580
Schroeder HA, Newby J, Schaefer A, Subramani B, Tubbs A, Forest MG, Miao E, Lai SK. (2020) LPS-binding IgG arrests actively motile Salmonella Typhimurium in gastrointestinal mucus. Mucosal Immunology. https://doi.org/10.1038/s41385-020-0267-9
Lai SK, McSweeney M, Pickles R (2020) Learning from past failures: challenges with monoclonal antibody therapies for COVID-19. J Controlled Release
Cruz-Teran C, Tiruthani K, McSweeney M, Ma A, Pickles R Lai SK, (2020) Challenges and opportunities for antiviral monoclonal antibodies as COVID-19 therapy. Advanced Drug Delivery Reviews